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Mol. Cell. Biol. doi:10.1128/MCB.00242-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Mir-17-5p Regulates Breast Cancer Cell Proliferation by Inhibiting Translation of AIB1 mRNA

Department of Biochemistry and Molecular Biology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030; Department of Molecular Pathology, The University of Texas M. D. Anderson Cancer Center, 7435 Fannin street, Houston, Texas 77054

^* To whom correspondence should be addressed. Email: ahossain{at}mdanderson.org,

	Abstract

MicroRNAs are an extensive family of 22-nucleotide-long noncoding RNAs expressed in a wide range of eukaryotes, including humans, and they are important in development and disease. We found that microRNA Mir-17-5p has extensive complementarity to the mRNA of amplified in breast cancer-1 (AIB1). Cell culture experiments showed that AIB1 expression was downregulated by Mir-17-5p, primarily through translational inhibition. Expression of Mir-17-5p was low in breast cancer cell lines. We also found that downregulation of AIB1 by Mir-17-5p resulted in decreased estrogen receptor-mediated as well as estrogen receptor-independent gene expression and decreased proliferation of breast cancer cells. Mir-17-5p also completely abrogated the IGF-1-mediated, anchorage-independent growth of breast cancer cells. Our results reveal that Mir-17-5p has a role as a tumor suppressor in breast cancer cells.

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