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Mol Cell Biol. 1985 December; 5(12): 3610-3616

Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.

G I Evan, G K Lewis, G Ramsay and J M Bishop

ABSTRACT

Six monoclonal antibodies have been isolated from mice immunized with synthetic peptide immunogens whose sequences are derived from that of the human c-myc gene product. Five of these antibodies precipitate p62c-myc from human cells, and three of these five also recognize the mouse c-myc gene product. None of the antibodies sees the chicken p110gag-myc protein. All six antibodies recognize immunoblotted p62c-myc. These reagents also provide the basis for an immunoblotting assay by which to quantitate p62c-myc in cells.

Mol Cell Biol. 1985 December; 5(12): 3610-3616




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