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Molecular and Cellular Biology, November 2006, p. 8191-8201, Vol. 26, No. 21
0270-7306/06/$08.00+0     doi:10.1128/MCB.00242-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Mir-17-5p Regulates Breast Cancer Cell Proliferation by Inhibiting Translation of AIB1 mRNA{triangledown}

Anwar Hossain,1* Macus T. Kuo,2 and Grady F. Saunders1,{dagger}

Department of Biochemistry and Molecular Biology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030,1 Department of Molecular Pathology, The University of Texas M. D. Anderson Cancer Center, 7435 Fannin Street, Houston, Texas 770542

Received 8 February 2006/ Returned for modification 3 April 2006/ Accepted 15 August 2006

MicroRNAs are an extensive family of ~22-nucleotide-long noncoding RNAs expressed in a wide range of eukaryotes, including humans, and they are important in development and disease. We found that microRNA Mir-17-5p has extensive complementarity to the mRNA of AIB1 (named for "amplified in breast cancer 1"). Cell culture experiments showed that AIB1 expression was downregulated by Mir-17-5p, primarily through translational inhibition. Expression of Mir-17-5p was low in breast cancer cell lines. We also found that downregulation of AIB1 by Mir-17-5p resulted in decreased estrogen receptor-mediated, as well as estrogen receptor-independent, gene expression and decreased proliferation of breast cancer cells. Mir-17-5p also completely abrogated the insulin-like growth factor 1-mediated, anchorage-independent growth of breast cancer cells. Our results reveal that Mir-17-5p has a role as a tumor suppressor in breast cancer cells.

* Corresponding author. Mailing address: Department of Molecular Pathology, Unit 89, The University of Texas M. D. Anderson Cancer Center, 7435 Fannin Street, Houston, TX 77054. Phone: (713) 834-6079. Fax: (713) 834-6084. E-mail: ahossain{at}mdanderson.org.

{triangledown} Published ahead of print on 28 August 2006.

{dagger} Deceased.

Molecular and Cellular Biology, November 2006, p. 8191-8201, Vol. 26, No. 21
0270-7306/06/$08.00+0     doi:10.1128/MCB.00242-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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