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Molecular and Cellular Biology, January 2001, p. 164-174, Vol. 21, No. 1
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.1.164-174.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

A Novel GATA Factor Transcriptionally Represses Yolk Protein Precursor Genes in the Mosquito Aedes aegypti via Interaction with the CtBP Corepressor

David Martín,1,dagger Maria-Dolors Piulachs,2 and Alexander S. Raikhel1,*

Department of Entomology and Program in Genetics, Michigan State University, East Lansing, Michigan 48824,1 and Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular de Barcelona, CID, CSIC, Barcelona, Spain2

Received 9 June 2000/Returned for modification 24 July 2000/Accepted 16 October 2000

In anautogenous mosquitoes, vitellogenesis, the key event in egg maturation, requires a blood meal. Consequently, mosquitoes are vectors of many devastating human diseases. An important adaptation for anautogenicity is the previtellogenic arrest (the state of arrest) preventing the activation of the yolk protein precursor (YPP) genes Vg and VCP prior to blood feeding. A novel GATA factor (AaGATAr) that recognizes GATA binding motifs (WGATAR) in the upstream region of the YPP genes serves as a transcriptional repressor at the state of arrest. Importantly, AaGATAr can override the 20-hydroxyecdysone transactivation of YPP genes, and its transcriptional repression involves the recruitment of CtBP, one of the universal corepressors. AaGATAr transcript is present only in the adult female fat body. Furthermore, in nuclear extracts of previtellogenic fat bodies with transcriptionally repressed YPP genes, there is a GATA binding protein forming a band with mobility similar to that of AaGATAr. The specific repression of YPP genes by AaGATAr in the fat body of the female mosquito during the state of arrest represents an important molecular adaptation for anautogenicity.

* Corresponding author. Mailing address: Department of Entomology and Program in Genetics, S-150 Plant Biology Building, Michigan State University, East Lansing, MI 48824. Phone: (517) 353-7144. Fax: (517) 353-3396. E-mail: araikhel{at}pilot.msu.edu.

dagger Present address: Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular de Barcelona, CID, CSIC, Barcelona, Spain.

Molecular and Cellular Biology, January 2001, p. 164-174, Vol. 21, No. 1
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.1.164-174.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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