This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Neff, M W
Right arrow Articles by Burke, D J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Neff, M W
Right arrow Articles by Burke, D J

 Previous Article  |  Next Article 

Mol Cell Biol. 1992 September; 12(9): 3857-3864

A delay in the Saccharomyces cerevisiae cell cycle that is induced by a dicentric chromosome and dependent upon mitotic checkpoints.

M W Neff and D J Burke

Department of Biology, University of Virginia, Charlottesville 22901.

ABSTRACT

Dicentric chromosomes are genetically unstable and depress the rate of cell division in Saccharomyces cerevisiae. We have characterized the effects of a conditionally dicentric chromosome on the cell division cycle by using microscopy, flow cytometry, and an assay for histone H1 kinase activity. Activating the dicentric chromosome induced a delay in the cell cycle after DNA replication and before anaphase. The delay occurred in the absence of RAD9, a gene required to arrest cell division in response to DNA damage. The rate of dicentric chromosome loss, however, was elevated in the rad9 mutant. A mutation in BUB2, a gene required for arrest of cell division in response to loss of microtubule function, diminished the delay. Both RAD9 and BUB2 appear to be involved in the cellular response to a dicentric chromosome, since the conditionally dicentric rad9 bub2 double mutant was highly inviable. We conclude that a dicentric chromosome results in chromosome breakage and spindle aberrations prior to nuclear division that normally activate mitotic checkpoints, thereby delaying the onset of anaphase.

Mol Cell Biol. 1992 September; 12(9): 3857-3864




This article has been cited by other articles:

  • Admire, A., Shanks, L., Danzl, N., Wang, M., Weier, U., Stevens, W., Hunt, E., Weinert, T. (2006). Cycles of chromosome instability are associated with a fragile site and are increased by defects in DNA replication and checkpoint controls in yeast. Genes Dev. 20: 159-173 [Abstract] [Full Text]  
  • Collins, K. A., Castillo, A. R., Tatsutani, S. Y., Biggins, S. (2005). De Novo Kinetochore Assembly Requires the Centromeric Histone H3 Variant. Mol. Biol. Cell 16: 5649-5660 [Abstract] [Full Text]  
  • Michel, J. J., McCarville, J. F., Xiong, Y. (2003). A Role for Saccharomyces cerevisiae Cul8 Ubiquitin Ligase in Proper Anaphase Progression. J. Biol. Chem. 278: 22828-22837 [Abstract] [Full Text]  
  • Thrower, D. A., Stemple, J., Yeh, E., Bloom, K. (2003). Nuclear oscillations and nuclear filament formation accompany single-strand annealing repair of a dicentric chromosome in Saccharomyces cerevisiae. J. Cell Sci. 116: 561-569 [Abstract] [Full Text]  
  • Maringele, L., Lydall, D. (2002). EXO1-dependent single-stranded DNA at telomeres activates subsets of DNA damage and spindle checkpoint pathways in budding yeast yku70Delta mutants. Genes Dev. 16: 1919-1933 [Abstract] [Full Text]  
  • Craven, R. J., Greenwell, P. W., Dominska, M., Petes, T. D. (2002). Regulation of Genome Stability by TEL1 and MEC1, Yeast Homologs of the Mammalian ATM and ATR Genes. Genetics 161: 493-507 [Abstract] [Full Text]  
  • Tavormina, P. A., Burke, D. J. (1998). Cell Cycle Arrest in cdc20 Mutants of Saccharomyces cerevisiae Is Independent of Ndc10p and Kinetochore Function but Requires a Subset of Spindle Checkpoint Genes. Genetics 148: 1701-1713 [Abstract] [Full Text]  
  • Juang, Y.-L., Huang, J., Peters, J.-M., McLaughlin, M. E., Tai, C.-Y., Pellman, D. (1997). APC-Mediated Proteolysis of Ase1 and the Morphogenesis of the Mitotic Spindle. Science 275: 1311-1314 [Abstract] [Full Text]  
  • Nicklas, R. B. (1997). How Cells Get the Right Chromosomes. Science 275: 632-637 [Abstract] [Full Text]  
  • Yang, S. S., Yeh, E., Salmon, E.D., Bloom, K. (1997). Identification of a Mid-anaphase Checkpoint in Budding Yeast. JCB 136: 345-354 [Abstract] [Full Text]  
  • Li, X, Nicklas, R. (1997). Tension-sensitive kinetochore phosphorylation and the chromosome distribution checkpoint in praying mantid spermatocytes. J. Cell Sci. 110: 537-545 [Abstract]  
  • Megee, P C, Morgan, B A, Smith, M M (1995). Histone H4 and the maintenance of genome integrity.. Genes Dev. 9: 1716-1727 [Abstract]  
  • Weinert, T A, Kiser, G L, Hartwell, L H (1994). Mitotic checkpoint genes in budding yeast and the dependence of mitosis on DNA replication and repair.. Genes Dev. 8: 652-665 [Abstract]  
  • Brock, J., Bloom, K (1994). A chromosome breakage assay to monitor mitotic forces in budding yeast. J. Cell Sci. 107: 891-902 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»