This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Semenza, G L Articles by Wang, G L Search for Related Content PubMed PubMed Citation Articles by Semenza, G L Articles by Wang, G L

 Previous Article  |  Next Article 

Mol Cell Biol. 1992 December; 12(12): 5447-5454

A nuclear factor induced by hypoxia via de novo protein synthesis binds to the human erythropoietin gene enhancer at a site required for transcriptional activation.

Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

ABSTRACT

We have identified a 50-nucleotide enhancer from the human erythropoietin gene 3'-flanking sequence which can mediate a sevenfold transcriptional induction in response to hypoxia when cloned 3' to a simian virus 40 promoter-chloramphenicol acetyltransferase reporter gene and transiently expressed in Hep3B cells. Nucleotides (nt) 1 to 33 of this sequence mediate sevenfold induction of reporter gene expression when present in two tandem copies compared with threefold induction when present in a single copy, suggesting that nt 34 to 50 bind a factor which amplifies the induction signal. DNase I footprinting demonstrated binding of a constitutive nuclear factor to nt 26 to 48. Mutagenesis studies revealed that nt 4 to 12 and 19 to 23 are essential for induction, as substitutions at either site eliminated hypoxia-induced expression. Electrophoretic mobility shift assays identified a nuclear factor which bound to a probe spanning nt 1 to 18 but not to a probe containing a mutation which eliminated enhancer function. Factor binding was induced by hypoxia, and its induction was sensitive to cycloheximide treatment. We have thus defined a functionally tripartite, 50-nt hypoxia-inducible enhancer which binds several nuclear factors, one of which is induced by hypoxia via de novo protein synthesis.

This article has been cited by other articles:

• Walczak-Drzewiecka, A., Ratajewski, M., Wagner, W., Dastych, J. (2008). HIF-1{alpha} Is Up-Regulated in Activated Mast Cells by a Process That Involves Calcineurin and NFAT. J. Immunol. 181: 1665-1672 [Abstract] [Full Text]  
• Lauzier, M.-C., Robitaille, G. A., Chan, D. A., Giaccia, A. J., Richard, D. E. (2008). (2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide (BiPS), a Matrix Metalloprotease Inhibitor, Is a Novel and Potent Activator of Hypoxia-Inducible Factors. Mol. Pharmacol. 74: 282-288 [Abstract] [Full Text]  
• Garcia-Ramirez, M., Hernandez, C., Simo, R. (2008). Expression of Erythropoietin and Its Receptor in the Human Retina: A comparative study of diabetic and nondiabetic subjects. Diabetes Care 31: 1189-1194 [Abstract] [Full Text]  
• Obara, N., Suzuki, N., Kim, K., Nagasawa, T., Imagawa, S., Yamamoto, M. (2008). Repression via the GATA box is essential for tissue-specific erythropoietin gene expression. Blood 111: 5223-5232 [Abstract] [Full Text]  
• Lee, K., Lynd, J. D., O'Reilly, S., Kiupel, M., McCormick, J. J., LaPres, J. J. (2008). The Biphasic Role of the Hypoxia-Inducible Factor Prolyl-4-Hydroxylase, PHD2, in Modulating Tumor-Forming Potential. Mol Cancer Res 6: 829-842 [Abstract] [Full Text]  
• Li, X., Lu, Y., Liang, K., Pan, T., Mendelsohn, J., Fan, Z. (2008). Requirement of hypoxia-inducible factor-1{alpha} down-regulation in mediating the antitumor activity of the anti-epidermal growth factor receptor monoclonal antibody cetuximab. Molecular Cancer Therapeutics 7: 1207-1217 [Abstract] [Full Text]  
• Wang, J. J., Zhang, S. X., Mott, R., Chen, Y., Knapp, R. R., Cao, W., Ma, J.-x. (2008). Anti-inflammatory effects of pigment epithelium-derived factor in diabetic nephropathy. Am. J. Physiol. Renal Physiol. 294: F1166-F1173 [Abstract] [Full Text]  
• Vaupel, P. (2008). Hypoxia and Aggressive Tumor Phenotype: Implications for Therapy and Prognosis. The Oncologist 13: 21-26 [Abstract] [Full Text]  
• Zhang, H., Bosch-Marce, M., Shimoda, L. A., Tan, Y. S., Baek, J. H., Wesley, J. B., Gonzalez, F. J., Semenza, G. L. (2008). Mitochondrial Autophagy Is an HIF-1-dependent Adaptive Metabolic Response to Hypoxia. J. Biol. Chem. 283: 10892-10903 [Abstract] [Full Text]  
• Shin, Y. C., Joo, C.-H., Gack, M. U., Lee, H.-R., Jung, J. U. (2008). Kaposi's Sarcoma-Associated Herpesvirus Viral IFN Regulatory Factor 3 Stabilizes Hypoxia-Inducible Factor-1{alpha} to Induce Vascular Endothelial Growth Factor Expression. Cancer Res. 68: 1751-1759 [Abstract] [Full Text]  
• Walmsley, S. R., McGovern, N. N., Whyte, M. K. B., Chilvers, E. R. (2008). The HIF/VHL Pathway: From Oxygen Sensing to Innate Immunity. Am. J. Respir. Cell Mol. Bio. 38: 251-255 [Abstract] [Full Text]  
• Gutsaeva, D. R., Carraway, M. S., Suliman, H. B., Demchenko, I. T., Shitara, H., Yonekawa, H., Piantadosi, C. A. (2008). Transient Hypoxia Stimulates Mitochondrial Biogenesis in Brain Subcortex by a Neuronal Nitric Oxide Synthase-Dependent Mechanism. J. Neurosci. 28: 2015-2024 [Abstract] [Full Text]  
• Flashman, E., Bagg, E. A. L., Chowdhury, R., Mecinovic, J., Loenarz, C., McDonough, M. A., Hewitson, K. S., Schofield, C. J. (2008). Kinetic Rationale for Selectivity toward N- and C-terminal Oxygen-dependent Degradation Domain Substrates Mediated by a Loop Region of Hypoxia-Inducible Factor Prolyl Hydroxylases. J. Biol. Chem. 283: 3808-3815 [Abstract] [Full Text]  
• Elser, M., Borsig, L., Hassa, P. O., Erener, S., Messner, S., Valovka, T., Keller, S., Gassmann, M., Hottiger, M. O. (2008). Poly(ADP-Ribose) Polymerase 1 Promotes Tumor Cell Survival by Coactivating Hypoxia-Inducible Factor-1-Dependent Gene Expression. Mol Cancer Res 6: 282-290 [Abstract] [Full Text]  
• Yang, L., Jiang, Y., Wu, S.F., Zhou, M.Y., Wu, Y.L., Chen, G.Q. (2008). CCAAT/enhancer-binding protein {alpha} antagonizes transcriptional activity of hypoxia-inducible factor 1 {alpha} with direct protein-protein interaction. Carcinogenesis 29: 291-298 [Abstract] [Full Text]  
• Hill, P., Shukla, D., Tran, M. G.B., Aragones, J., Cook, H. T., Carmeliet, P., Maxwell, P. H. (2008). Inhibition of Hypoxia Inducible Factor Hydroxylases Protects Against Renal Ischemia-Reperfusion Injury. J. Am. Soc. Nephrol. 19: 39-46 [Abstract] [Full Text]  
• Page, E. L., Chan, D. A., Giaccia, A. J., Levine, M., Richard, D. E. (2008). Hypoxia-inducible Factor-1{alpha} Stabilization in Nonhypoxic Conditions: Role of Oxidation and Intracellular Ascorbate Depletion. Mol. Biol. Cell 19: 86-94 [Abstract] [Full Text]  
• Nangaku, M., Izuhara, Y., Takizawa, S., Yamashita, T., Fujii-Kuriyama, Y., Ohneda, O., Yamamoto, M., van Ypersele de Strihou, C., Hirayama, N., Miyata, T. (2007). A Novel Class of Prolyl Hydroxylase Inhibitors Induces Angiogenesis and Exerts Organ Protection Against Ischemia. Arterioscler. Thromb. Vasc. Bio. 27: 2548-2554 [Abstract] [Full Text]  
• Zhang, P., Tchou-Wong, K.-M., Costa, M. (2007). Egr-1 Mediates Hypoxia-Inducible Transcription of the NDRG1 Gene through an Overlapping Egr-1/Sp1 Binding Site in the Promoter. Cancer Res. 67: 9125-9133 [Abstract] [Full Text]  
• Dehne, N., Kerkweg, U., Otto, T., Fandrey, J. (2007). The HIF-1 response to simulated ischemia in mouse skeletal muscle cells neither enhances glycolysis nor prevents myotube cell death. Am. J. Physiol. Regul. Integr. Comp. Physiol. 293: R1693-R1701 [Abstract] [Full Text]  
• Baek, J. H., Liu, Y. V., McDonald, K. R., Wesley, J. B., Hubbi, M. E., Byun, H., Semenza, G. L. (2007). Spermidine/Spermine-N1-Acetyltransferase 2 Is an Essential Component of the Ubiquitin Ligase Complex That Regulates Hypoxia-inducible Factor 1{alpha}. J. Biol. Chem. 282: 23572-23580 [Abstract] [Full Text]  
• Lauzier, M.-C., Page, E. L., Michaud, M. D., Richard, D. E. (2007). Differential Regulation of Hypoxia-Inducible Factor-1 through Receptor Tyrosine Kinase Transactivation in Vascular Smooth Muscle Cells. Endocrinology 148: 4023-4031 [Abstract] [Full Text]  
• WESTRA, J., BROUWER, E., BOS, R., POSTHUMUS, M. D., DOORNBOS-VAN DER MEER, B., KALLENBERG, C. G.M., LIMBURG, P. C. (2007). Regulation of Cytokine-Induced HIF-1{alpha} Expression in Rheumatoid Synovial Fibroblasts. Ann. N. Y. Acad. Sci. 1108: 340-348 [Abstract] [Full Text]  
• Ronkainen, V.-P., Ronkainen, J. J., Hanninen, S. L., Leskinen, H., Ruas, J. L., Pereira, T., Poellinger, L., Vuolteenaho, O., Tavi, P. (2007). Hypoxia inducible factor regulates the cardiac expression and secretion of apelin. FASEB J. 21: 1821-1830 [Abstract] [Full Text]  
• Choi, K. J., Piao, Y. J., Lim, M. J., Kim, J. H., Ha, J., Choe, W., Kim, S. S. (2007). Overexpressed Cyclophilin A in Cancer Cells Renders Resistance to Hypoxia- and Cisplatin-Induced Cell Death. Cancer Res. 67: 3654-3662 [Abstract] [Full Text]  
• Madeddu, P., Emanueli, C. (2007). Switching on Reparative Angiogenesis: Essential Role of the Vascular Erythropoietin Receptor. Circ. Res. 100: 599-601 [Full Text]  
• Nakano, M., Satoh, K., Fukumoto, Y., Ito, Y., Kagaya, Y., Ishii, N., Sugamura, K., Shimokawa, H. (2007). Important Role of Erythropoietin Receptor to Promote VEGF Expression and Angiogenesis in Peripheral Ischemia in Mice. Circ. Res. 100: 662-669 [Abstract] [Full Text]  
• Semenza, G. L. (2006). Baffled by Bafilomycin: An Anticancer Agent That Induces Hypoxia-Inducible Factor-1{alpha} Expression. Mol. Pharmacol. 70: 1841-1843 [Abstract] [Full Text]  
• Jean, J.-C., Rich, C. B., Joyce-Brady, M. (2006). Hypoxia results in an HIF-1-dependent induction of brain-specific aldolase C in lung epithelial cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 291: L950-L956 [Abstract] [Full Text]  
• Rissanen, E., Tranberg, H. K., Nikinmaa, M. (2006). Oxygen availability regulates metabolism and gene expression in trout hepatocyte cultures. Am. J. Physiol. Regul. Integr. Comp. Physiol. 291: R1507-R1515 [Abstract] [Full Text]  
• Walmsley, S. R., Cowburn, A. S., Clatworthy, M. R., Morrell, N. W., Roper, E. C., Singleton, V., Maxwell, P., Whyte, M. K. B., Chilvers, E. R. (2006). Neutrophils from patients with heterozygous germline mutations in the von Hippel Lindau protein (pVHL) display delayed apoptosis and enhanced bacterial phagocytosis. Blood 108: 3176-3178 [Abstract] [Full Text]  
• Koulmann, N., Novel-Chate, V., Peinnequin, A., Chapot, R., Serrurier, B., Simler, N., Richard, H., Ventura-Clapier, R., Bigard, X. (2006). Cyclosporin A Inhibits Hypoxia-induced Pulmonary Hypertension and Right Ventricle Hypertrophy. Am. J. Respir. Crit. Care Med. 174: 699-705 [Abstract] [Full Text]  
• Yoon, D., Pastore, Y. D., Divoky, V., Liu, E., Mlodnicka, A. E., Rainey, K., Ponka, P., Semenza, G. L., Schumacher, A., Prchal, J. T. (2006). Hypoxia-inducible Factor-1 Deficiency Results in Dysregulated Erythropoiesis Signaling and Iron Homeostasis in Mouse Development. J. Biol. Chem. 281: 25703-25711 [Abstract] [Full Text]  
• Semenza, G. L. (2006). Regulation of physiological responses to continuous and intermittent hypoxia by hypoxia-inducible factor 1. Exp Physiol 91: 803-806 [Abstract] [Full Text]  
• Guzy, R. D., Schumacker, P. T. (2006). Oxygen sensing by mitochondria at complex III: the paradox of increased reactive oxygen species during hypoxia. Exp Physiol 91: 807-819 [Abstract] [Full Text]  
• McMahon, S., Charbonneau, M., Grandmont, S., Richard, D. E., Dubois, C. M. (2006). Transforming Growth Factor beta1 Induces Hypoxia-inducible Factor-1 Stabilization through Selective Inhibition of PHD2 Expression. J. Biol. Chem. 281: 24171-24181 [Abstract] [Full Text]  
• Grimmer, C., Balbus, N., Lang, U., Aigner, T., Cramer, T., Muller, L., Swoboda, B., Pfander, D. (2006). Regulation of Type II Collagen Synthesis during Osteoarthritis by Prolyl-4-Hydroxylases: Possible Influence of Low Oxygen Levels. Am. J. Pathol. 169: 491-502 [Abstract] [Full Text]  
• Wang, C., Weihrauch, D., Schwabe, D. A., Bienengraeber, M., Warltier, D. C., Kersten, J. R., Pratt, P. F. Jr, Pagel, P. S. (2006). Extracellular signal-regulated kinases trigger isoflurane preconditioning concomitant with upregulation of hypoxia-inducible factor-1alpha and vascular endothelial growth factor expression in rats.. Anesth. Analg. 103: 281-8, table of contents [Abstract] [Full Text]  
• Haque, M., Wang, V., Davis, D. A., Zheng, Z.-M., Yarchoan, R. (2006). Genetic Organization and Hypoxic Activation of the Kaposi's Sarcoma-Associated Herpesvirus ORF34-37 Gene Cluster. J. Virol. 80: 7037-7051 [Abstract] [Full Text]  
• Aprelikova, O., Wood, M., Tackett, S., Chandramouli, G. V.R., Barrett, J. C. (2006). Role of ETS Transcription Factors in the Hypoxia-Inducible Factor-2 Target Gene Selection. Cancer Res. 66: 5641-5647 [Abstract] [Full Text]  
• Luo, Y., Jiang, C., Belanger, A. J., Akita, G. Y., Wadsworth, S. C., Gregory, R. J., Vincent, K. A. (2006). A Constitutively Active Hypoxia-Inducible Factor-1{alpha}/VP16 Hybrid Factor Activates Expression of the Human B-Type Natriuretic Peptide Gene. Mol. Pharmacol. 69: 1953-1962 [Abstract] [Full Text]  
• Mense, S. M., Sengupta, A., Zhou, M., Lan, C., Bentsman, G., Volsky, D. J., Zhang, L. (2006). Gene expression profiling reveals the profound upregulation of hypoxia-responsive genes in primary human astrocytes. Physiol. Genomics 25: 435-449 [Abstract] [Full Text]  
• Tarn, C., Skorobogatko, Y. V., Taguchi, T., Eisenberg, B., von Mehren, M., Godwin, A. K. (2006). Therapeutic Effect of Imatinib in Gastrointestinal Stromal Tumors: AKT Signaling Dependent and Independent Mechanisms.. Cancer Res. 66: 5477-5486 [Abstract] [Full Text]  
• Resnik, E., Herron, J., Fu, R., Ivy, D. D., Cornfield, D. N. (2006). Oxygen tension modulates the expression of pulmonary vascular BKCa channel {alpha}- and beta-subunits. Am. J. Physiol. Lung Cell. Mol. Physiol. 290: L761-L768 [Abstract] [Full Text]  
• Zhou, J., Kohl, R., Herr, B., Frank, R., Brune, B. (2006). Calpain Mediates a von Hippel-Lindau Protein-independent Destruction of Hypoxia-inducible Factor-1{alpha}. Mol. Biol. Cell 17: 1549-1558 [Abstract] [Full Text]  
• Satoh, K., Kagaya, Y., Nakano, M., Ito, Y., Ohta, J., Tada, H., Karibe, A., Minegishi, N., Suzuki, N., Yamamoto, M., Ono, M., Watanabe, J., Shirato, K., Ishii, N., Sugamura, K., Shimokawa, H. (2006). Important Role of Endogenous Erythropoietin System in Recruitment of Endothelial Progenitor Cells in Hypoxia-Induced Pulmonary Hypertension in Mice. Circulation 113: 1442-1450 [Abstract] [Full Text]  
• Rissanen, E., Tranberg, H. K., Sollid, J., Nilsson, G. E., Nikinmaa, M. (2006). Temperature regulates hypoxia-inducible factor-1 (HIF-1) in a poikilothermic vertebrate, crucian carp (Carassius carassius). J. Exp. Biol. 209: 994-1003 [Abstract] [Full Text]  
• Dimova, E. Y., Kietzmann, T. (2006). Cell Type-dependent Regulation of the Hypoxia-responsive Plasminogen Activator Inhibitor-1 Gene by Upstream Stimulatory Factor-2. J. Biol. Chem. 281: 2999-3005 [Abstract] [Full Text]  
• Kajimura, S., Aida, K., Duan, C. (2006). Understanding Hypoxia-Induced Gene Expression in Early Development: In Vitro and In Vivo Analysis of Hypoxia-Inducible Factor 1-Regulated Zebra Fish Insulin-Like Growth Factor Binding Protein 1 Gene Expression. Mol. Cell. Biol. 26: 1142-1155 [Abstract] [Full Text]  
• Csiki, I., Yanagisawa, K., Haruki, N., Nadaf, S., Morrow, J. D., Johnson, D. H., Carbone, D. P. (2006). Thioredoxin-1 Modulates Transcription of Cyclooxygenase-2 via Hypoxia-Inducible Factor-1{alpha} in Non-Small Cell Lung Cancer. Cancer Res. 66: 143-150 [Abstract] [Full Text]  
• Sairanen, T., Karjalainen-Lindsberg, M.-L., Paetau, A., Ijas, P., Lindsberg, P. J. (2006). Apoptosis dominant in the periinfarct area of human ischaemic stroke--a possible target of antiapoptotic treatments. Brain 129: 189-199 [Abstract] [Full Text]  
• Nangaku, M. (2006). Chronic Hypoxia and Tubulointerstitial Injury: A Final Common Pathway to End-Stage Renal Failure. J. Am. Soc. Nephrol. 17: 17-25 [Abstract] [Full Text]  
• Schepens, B., Tinton, S. A., Bruynooghe, Y., Beyaert, R., Cornelis, S. (2005). The polypyrimidine tract-binding protein stimulates HIF-1{alpha} IRES-mediated translation during hypoxia. Nucleic Acids Res 33: 6884-6894 [Abstract] [Full Text]  
• Chang, T.-C., Huang, C.-J., Tam, K., Chen, S.-F., Tan, K. T., Tsai, M.-S., Lin, T.-N., Shyue, S.-K. (2005). Stabilization of Hypoxia-inducible Factor-1{alpha} by Prostacyclin under Prolonged Hypoxia via Reducing Reactive Oxygen Species Level in Endothelial Cells. J. Biol. Chem. 280: 36567-36574 [Abstract] [Full Text]  
• Sears, J. E., Hoppe, G. (2005). Triamcinolone Acetonide Destabilizes VEGF mRNA in Muller Cells under Continuous Cobalt Stimulation. IOVS 46: 4336-4341 [Abstract] [Full Text]  
• Hayashi, M., Sakata, M., Takeda, T., Tahara, M., Yamamoto, T., Okamoto, Y., Minekawa, R., Isobe, A., Ohmichi, M., Tasaka, K., Murata, Y. (2005). Up-Regulation of c-met Protooncogene Product Expression through Hypoxia-Inducible Factor-1{alpha} Is Involved in Trophoblast Invasion under Low-Oxygen Tension. Endocrinology 146: 4682-4689 [Abstract] [Full Text]  
• Winter, S. C., Shah, K. A., Campo, L., Turley, H., Leek, R., Corbridge, R. J., Cox, G. J., Harris, A. L. (2005). Relation of Erythropoietin and Erythropoietin Receptor Expression to Hypoxia and Anemia in Head and Neck Squamous Cell Carcinoma. Clin. Cancer Res. 11: 7614-7620 [Abstract] [Full Text]  
• Pollard, M., Suckow, M. A. (2005). Hormone-Refractory Prostate Cancer in the Lobund-Wistar Rat. Exp. Biol. Med. 230: 520-526 [Abstract] [Full Text]  
• Watanabe, D., Suzuma, K., Matsui, S., Kurimoto, M., Kiryu, J., Kita, M., Suzuma, I., Ohashi, H., Ojima, T., Murakami, T., Kobayashi, T., Masuda, S., Nagao, M., Yoshimura, N., Takagi, H. (2005). Erythropoietin as a Retinal Angiogenic Factor in Proliferative Diabetic Retinopathy. NEJM 353: 782-792 [Abstract] [Full Text]  
• Zhang, X., Li, J., Sejas, D. P., Pang, Q. (2005). Hypoxia-reoxygenation induces premature senescence in FA bone marrow hematopoietic cells. Blood 106: 75-85 [Abstract] [Full Text]  
• Zhou, S., Lechpammer, S., Greenberger, J. S., Glowacki, J. (2005). Hypoxia Inhibition of Adipocytogenesis in Human Bone Marrow Stromal Cells Requires Transforming Growth Factor-{beta}/Smad3 Signaling. J. Biol. Chem. 280: 22688-22696 [Abstract] [Full Text]  
• Phillips, R. J., Mestas, J., Gharaee-Kermani, M., Burdick, M. D., Sica, A., Belperio, J. A., Keane, M. P., Strieter, R. M. (2005). Epidermal Growth Factor and Hypoxia-induced Expression of CXC Chemokine Receptor 4 on Non-small Cell Lung Cancer Cells Is Regulated by the Phosphatidylinositol 3-Kinase/PTEN/AKT/Mammalian Target of Rapamycin Signaling Pathway and Activation of Hypoxia Inducible Factor-1{alpha}. J. Biol. Chem. 280: 22473-22481 [Abstract] [Full Text]  
• La Padula, P., Costa, L. E. (2005). Effect of sustained hypobaric hypoxia during maturation and aging on rat myocardium. I. Mechanical activity. J. Appl. Physiol. 98: 2363-2369 [Abstract] [Full Text]  
• YANG, D.-I, CHEN, S.-D., YIN, J.-H., HSU, C. Y. (2005). S-Nitrosoglutathione and Hypoxia-Inducible Factor-1 Confer Chemoresistance against Carbamoylating Cytotoxicity of BCNU in Rat C6 Glioma Cells. Ann. N. Y. Acad. Sci. 1042: 229-234 [Abstract] [Full Text]  
• Gong, Y., Agani, F. H. (2005). Oligomycin inhibits HIF-1{alpha} expression in hypoxic tumor cells. Am. J. Physiol. Cell Physiol. 288: C1023-C1029 [Abstract] [Full Text]  
• Nikinmaa, M., Rees, B. B. (2005). Oxygen-dependent gene expression in fishes. Am. J. Physiol. Regul. Integr. Comp. Physiol. 288: R1079-R1090 [Abstract] [Full Text]  
• Wang, V., Davis, D. A., Haque, M., Huang, L. E., Yarchoan, R. (2005). Differential Gene Up-Regulation by Hypoxia-Inducible Factor-1{alpha} and Hypoxia-Inducible Factor-2{alpha} in HEK293T Cells. Cancer Res. 65: 3299-3306 [Abstract] [Full Text]  
• Kaur, B., Khwaja, F. W., Severson, E. A., Matheny, S. L., Brat, D. J., Van Meir, E. G. (2005). Hypoxia and the hypoxia-inducible-factor pathway in glioma growth and angiogenesis. Neuro Oncol 7: 134-153 [Abstract]  
• Piret, J.-P., Minet, E., Cosse, J.-P., Ninane, N., Debacq, C., Raes, M., Michiels, C. (2005). Hypoxia-inducible Factor-1-dependent Overexpression of Myeloid Cell Factor-1 Protects Hypoxic Cells against tert-Butyl Hydroperoxide-induced Apoptosis. J. Biol. Chem. 280: 9336-9344 [Abstract] [Full Text]  
• Hayashi, M., Sakata, M., Takeda, T., Tahara, M., Yamamoto, T., Minekawa, R., Isobe, A., Tasaka, K., Murata, Y. (2005). Hypoxia Up-Regulates Hypoxia-Inducible Factor-1{alpha} Expression through RhoA Activation in Trophoblast Cells. J. Clin. Endocrinol. Metab. 90: 1712-1719 [Abstract] [Full Text]  
• McMahon, S., Grondin, F., McDonald, P. P., Richard, D. E., Dubois, C. M. (2005). Hypoxia-enhanced Expression of the Proprotein Convertase Furin Is Mediated by Hypoxia-inducible Factor-1: IMPACT ON THE BIOACTIVATION OF PROPROTEINS. J. Biol. Chem. 280: 6561-6569 [Abstract] [Full Text]  
• Wachsberger, P. R., Burd, R., Marero, N., Daskalakis, C., Ryan, A., McCue, P., Dicker, A. P. (2005). Effect of the Tumor Vascular-Damaging Agent, ZD6126, on the Radioresponse of U87 Glioblastoma. Clin. Cancer Res. 11: 835-842 [Abstract] [Full Text]  
• Nakano, Y., Imagawa, S., Matsumoto, K., Stockmann, C., Obara, N., Suzuki, N., Doi, T., Kodama, T., Takahashi, S., Nagasawa, T., Yamamoto, M. (2004). Oral administration of K-11706 inhibits GATA binding activity, enhances hypoxia-inducible factor 1 binding activity, and restores indicators in an in vivo mouse model of anemia of chronic disease. Blood 104: 4300-4307 [Abstract] [Full Text]  
• Box, A. H., Demetrick, D. J. (2004). Cell cycle kinase inhibitor expression and hypoxia-induced cell cycle arrest in human cancer cell lines. Carcinogenesis 25: 2325-2335 [Abstract] [Full Text]  
• Wang, F., Zhang, R., Beischlag, T. V., Muchardt, C., Yaniv, M., Hankinson, O. (2004). Roles of Brahma and Brahma/SWI2-Related Gene 1 in Hypoxic Induction of the Erythropoietin Gene. J. Biol. Chem. 279: 46733-46741 [Abstract] [Full Text]  
• Vaupel, P. (2004). The Role of Hypoxia-Induced Factors in Tumor Progression. The Oncologist 9: 10-17 [Abstract] [Full Text]  
• Farrell, F., Lee, A. (2004). The Erythropoietin Receptor and Its Expression in Tumor Cells and Other Tissues. The Oncologist 9: 18-30 [Abstract] [Full Text]  
• Hayashi, M., Sakata, M., Takeda, T., Yamamoto, T., Okamoto, Y., Sawada, K., Kimura, A., Minekawa, R., Tahara, M., Tasaka, K., Murata, Y. (2004). Induction of glucose transporter 1 expression through hypoxia-inducible factor 1{alpha} under hypoxic conditions in trophoblast-derived cells. J Endocrinol 183: 145-154 [Abstract] [Full Text]  
• Fukasawa, M., Tsuchiya, T., Takayama, E., Shinomiya, N., Uyeda, K., Sakakibara, R., Seki, S. (2004). Identification and Characterization of the Hypoxia-Responsive Element of the Human Placental 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase Gene. J Biochem 136: 273-277 [Abstract] [Full Text]  
• Ma, Y., Freitag, P., Zhou, J., Brune, B., Frede, S., Fandrey, J. (2004). Thyroid hormone induces erythropoietin gene expression through augmented accumulation of hypoxia-inducible factor-1. Am. J. Physiol. Regul. Integr. Comp. Physiol. 287: R600-R607 [Abstract] [Full Text]  
• Reynolds, P. R., Mucenski, M. L., Le Cras, T. D., Nichols, W. C., Whitsett, J. A. (2004). Midkine Is Regulated by Hypoxia and Causes Pulmonary Vascular Remodeling. J. Biol. Chem. 279: 37124-37132 [Abstract] [Full Text]  
• Gorr, T. A., Cahn, J. D., Yamagata, H., Bunn, H. F. (2004). Hypoxia-induced Synthesis of Hemoglobin in the Crustacean Daphnia magna Is Hypoxia-inducible Factor-dependent. J. Biol. Chem. 279: 36038-36047 [Abstract] [Full Text]  
• Gorr, T. A., Tomita, T., Wappner, P., Bunn, H. F. (2004). Regulation of Drosophila Hypoxia-inducible Factor (HIF) Activity in SL2 Cells: IDENTIFICATION OF A HYPOXIA-INDUCED VARIANT ISOFORM OF THE HIF{alpha} HOMOLOG GENE similar. J. Biol. Chem. 279: 36048-36058 [Abstract] [Full Text]  
• Marti, H. H. (2004). Erythropoietin and the hypoxic brain. J. Exp. Biol. 207: 3233-3242 [Abstract] [Full Text]  
• Semenza, G. L. (2004). Hydroxylation of HIF-1: Oxygen Sensing at the Molecular Level. Physiology 19: 176-182 [Abstract] [Full Text]  
• Kinoshita, K., Kikuchi, Y., Sasakura, Y., Suzuki, M., Fujii-Kuriyama, Y., Sogawa, K. (2004). Altered DNA binding specificity of Arnt by selection of partner bHLH-PAS proteins. Nucleic Acids Res 32: 3169-3179 [Abstract] [Full Text]  
• Fandrey, J. (2004). Oxygen-dependent and tissue-specific regulation of erythropoietin gene expression. Am. J. Physiol. Regul. Integr. Comp. Physiol. 286: R977-R988 [Abstract] [Full Text]  
• Abraham, D., Krenn, K., Seebacher, G., Paulus, P., Klepetko, W., Aharinejad, S. (2004). Upregulated hypoxia-inducible factor-1 DNA binding activity to the vascular endothelial growth factor-A promoter mediates increased vascular permeability in donor lung grafts. Ann. Thorac. Surg. 77: 1751-1755 [Abstract] [Full Text]  
• Zelzer, E., Mamluk, R., Ferrara, N., Johnson, R. S., Schipani, E., Olsen, B. R. (2004). VEGFA is necessary for chondrocyte survival during bone development. Development 131: 2161-2171 [Abstract] [Full Text]  
• Semenza, G. L. (2004). O2-regulated gene expression: transcriptional control of cardiorespiratory physiology by HIF-1. J. Appl. Physiol. 96: 1173-1177 [Abstract] [Full Text]  
• Cowen, R. L., Williams, K. J., Chinje, E. C., Jaffar, M., Sheppard, F. C. D., Telfer, B. A., Wind, N. S., Stratford, I. J. (2004). Hypoxia Targeted Gene Therapy to Increase the Efficacy of Tirapazamine as an Adjuvant to Radiotherapy: Reversing Tumor Radioresistance and Effecting Cure. Cancer Res. 64: 1396-1402 [Abstract] [Full Text]  
• Chapman-Smith, A., Lutwyche, J. K., Whitelaw, M. L. (2004). Contribution of the Per/Arnt/Sim (PAS) Domains to DNA Binding by the Basic Helix-Loop-Helix PAS Transcriptional Regulators. J. Biol. Chem. 279: 5353-5362 [Abstract] [Full Text]  
• Javaheri, S., Abraham, W. T., Brown, C., Nishiyama, H., Giesting, R., Wagoner, L. E. (2004). Prevalence of obstructive sleep apnoea and periodic limb movement in 45 subjects with heart transplantation. Eur Heart J 25: 260-266 [Abstract] [Full Text]  
• Seta, K. A., Millhorn, D. E. (2004). Functional genomics approach to hypoxia signaling. J. Appl. Physiol. 96: 765-773 [Abstract] [Full Text]  
• Walmsley, S R, Sheares, K K K, Sobolewski, A, Morrell, N W, Chilvers, E R (2004). New insights into oxygen sensing at a cellular level. Thorax 59: 90-92 [Full Text]  
• Blouin, C. C., Page, E. L., Soucy, G. M., Richard, D. E. (2004). Hypoxic gene activation by lipopolysaccharide in macrophages: implication of hypoxia-inducible factor 1{alpha}. Blood 103: 1124-1130 [Abstract] [Full Text]  
• Roth, U., Curth, K., Unterman, T. G., Kietzmann, T. (2004). The Transcription Factors HIF-1 and HNF-4 and the Coactivator p300 Are Involved in Insulin-regulated Glucokinase Gene Expression via the Phosphatidylinositol 3-Kinase/Protein Kinase B Pathway. J. Biol. Chem. 279: 2623-2631 [Abstract] [Full Text]  
• Dame, C., Sola, M. C., Lim, K.-C., Leach, K. M., Fandrey, J., Ma, Y., Knopfle, G., Engel, J. D., Bungert, J. (2004). Hepatic Erythropoietin Gene Regulation by GATA-4. J. Biol. Chem. 279: 2955-2961 [Abstract] [Full Text]  
• Kim, J.-Y., Ahn, H.-J., Ryu, J.-H., Suk, K., Park, J.-H. (2004). BH3-only Protein Noxa Is a Mediator of Hypoxic Cell Death Induced by Hypoxia-inducible Factor 1{alpha}. J. Exp. Med. 199: 113-124 [Abstract] [Full Text]  
• Maschek, G., Savaraj, N., Priebe, W., Braunschweiger, P., Hamilton, K., Tidmarsh, G. F., De Young, L. R., Lampidis, T. J. (2004). 2-Deoxy-D-glucose Increases the Efficacy of Adriamycin and Paclitaxel in Human Osteosarcoma and Non-Small Cell Lung Cancers In Vivo. Cancer Res. 64: 31-34 [Abstract] [Full Text]