MCB Accepts, published online ahead of print on 26 October 2009

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Mol. Cell. Biol. doi:10.1128/MCB.00645-09
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Friend of Prmt1, a novel chromatin target of protein arginine methyltransferases

Thamar Bryn van Dijk, Nynke Gillemans, Claudia Stein, Pavlos Fanis, Jeroen Demmers, Mariëtte van de Corput, Jeroen Essers, Frank Grosveld, Uta-Maria Bauer, and Sjaak Philipsen^*

Department of Cell Biology, Erasmus MC, 3015 GE Rotterdam, the Netherlands; Institute for Molecular Biology and Tumor Research, Philipps-University Marburg, 35032 Marburg, Germany; Netherlands Proteomics Centre, Erasmus MC, 3015 GE Rotterdam, the Netherlands; Department of Genetics, Erasmus MC, 3015 GE Rotterdam, the Netherlands

^* To whom correspondence should be addressed. Email: j.philipsen{at}erasmusmc.nl.

We describe the isolation and characterization of Friend of Prmt1 (Fop), a novel chromatin target of protein arginine methyltransferases. Human Fop is encoded by C1orf77, a gene of previously unknown function. We show that Fop is tightly associated with chromatin, and that it is modified by both asymmetric- and symmetric arginine methylation in vivo. Furthermore, Fop plays an important role in the ligand-dependent activation of estrogen receptor target genes including TFF1 (pS2). Fop depletion results in an almost complete block of estradiol-induced promoter occupancy by the estrogen receptor. Our data indicate that Fop recruitment to the promoter is an early critical event in activation of estradiol-dependent transcription.